Molecularly Imprinted Polymer (MIP) columns for use in conjunction with an HPLC for detection of patulin in apple juice and apple purée.
The procedure is based on molecularly imprinted polymer (MIP) technology, which makes the test specific, sensitive, rapid and simple to perform.
|Art. no.||P250 / P250B|
|Test format||10 wide format MIP columns (P250).|
50 wide format MIP columns (P250B).
|Sample preparation||The columns contain a molecularly imprinted polymer specific to the toxin of interest. Following extraction of the toxin the sample extract is centrifuged, filtered, and passed through the MIP column. Any toxin which is present in the sample is retained by the MIP within the column. The column is washed to remove unbound material and the toxin is then released from the column following elution with solvent. The eluate is collected prior to analysis by HPLC.|
|Detection limit||0.06 ng.|
|Shelf life||3 years at 21 - 25 °C.|
|Sensitivity||Low % CV, excellent recoveries and detection limits.|
|Rapid||Clean up in 45 minutes prior to detection by HPLC.|
|Robust||Can be stored at room temperature.|
|Reliable||Uses a MIP to selectively isolate and concentrate patulin.|
|Adaptable||The columns are suitable for testing apple juice and apple purée.|
|Extraction solvents||Acetic acid or pectinase.|
|Additional information||Patulin standards and pectinase are available from R-Biopharm.|
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