The Danish company, RefLab, became a member of the R-Biopharm group on January 1, 2010. As a supplier of test kits for the detection of autoreactive urticaria and histamine release assays for the detection of type I allergies, RefLab is expanding R-Biopharm’s product portfolio in an ideal manner.
A glass fiber matrix for selective histamine binding, which was developed by RefLab, is the underlying basis of both tests. The basic principle of both tests is the provocation of basophile leukocytes, which release Histamine as a result. The released histamine is bound to the glass fiber matrix and can at a later date be dissolved away and measured fluorometrically in the high sensitive and specifically developed HISTAREADERTM 501.
Histamin Release Test
The Histamine Release assay can be used for diagnosing patients who are allergic to foodstuffs, drugs and insect venom. Allergies to other allergens can also be detected with this test system. To do this the patients´ blood must be mixed with the relevant allergens and incubated in a glass fiber coated well within 24 hours after blood withdrawal. After incubation the plate is washed. Now the histamine is bound to the glass fiber matrix permanently and the plate can be sent to the reference laboratory to measure the amount of released histamine.
In Germany the plates can be sent to the “Institute of Dermatology and Allergology” of the Charité in Berlin, Department of Prof. Maurer. The addresses for reference laboratories in other countries can be requested from R-Biopharm AG.
Histamin Release Urticaria test
Autoimmune disease is the root cause of approximately 30 – 40 % of chronic urticaria cases. These patients develop antibodies against the human Immunoglobulin E, or against the receptors FceRI, which bind to the IgE antibodies, or receptors on the surface of mast cells or basophiles inducing them to release histamine.
The HR urticaria assay requires only the serum of the patient, which is incubated together with basophile donor cells. If the serum contains corresponding antibodies the donor cells will react with releasing of histamine. The ratio of released histamine to the total content of histamine in the donor cells is determined, and if the ratio is higher than 16.5 % the result is regarded as positive.
This test can only be performed in reference laboratories. In Germany the HR urticaria test is also performed in the “Institute of Dermatology and Allergology” of the Charité in Berlin, Department of Prof. Maurer. If you look for a reference laboratory in another country please contact the Product Manager for Allergy Diagnostics at R-Biopharm AG.
For more detailed information please contact the Product Manager for Allergy Diagnostics at R-Biopharm AG or visit RefLab’s NEW website